| Gene name: | SUPT5H |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | RKO,HT29 |
| Gene ID: | 6829 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 26418880 |
| Experiment: | SA-β-gal activity assay//Colony formation assay//Knockdown |
| Description: | A higher number of senescent cells was observed in SUPT5H-knockdown cells (shSUPT5H) compared to that observed in wild-type cells or negative control cells (shNSC);Colony formation assays showed that the inhibition of SUPT5H expression effectively suppressed the colony formation efficiency of transfected cells in both monolayer cultures and soft agar, compared to that observed in wild-type cells and negative control cells (p < 0.05). |
| Target gene: | HTERT |
| Official symbol(s): | HTERT |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Flow cytometry//Immunostaining |
| Target gene description: | The results showed that inhibition of SPT5 expression by SUPT5H-specific shRNA attenuated hTERT promoter-driven GFP expression in RKO and HT29 cells transfected with hTERT promoter-driven GFP vector, whereas no obvious effect on CMV promoter-driven GFP expression was observed. Representative graphs of fluorescence-activated cell sorting (FACS) .These results indicated that SPT5 is essential for hTERT promoter activity and SUPT5H transcriptionally activated hTERT promoter-driven gene expression in human colon cancer cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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