| Gene name: | AKT1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BJ-T |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//SAHF//Cell counting |
| Description: | Cells expressing activated AKT isoforms and H-RASV12 showed a significant increase in SA-β-GAL and cell size, indicating that AKT activation is sufficient to induce cellular senescence. The formation of senescence-associated heterochromatic foci (SAHF), a marker of RAS- and DNA damage-induced senescence;We found that AKT rapidly induced proliferative arrest. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Immunostaining//Knockdown//DAPI staining |
| Target gene description: | A 45% increase in p53 synthesis was observed in the 30 min pulse period in myr-AKT-expressing cells compared with control cells.SA-β-GAL positivity was significantly reduced in BJ-T-myr-AKT/p53 stable knockdown cells as compared with control BJ-T-myr-AKT cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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