| Gene name: | CD38 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NPC,5-8F |
| Gene ID: | 952 |
| Category: | protein coding |
| Phenotype: | Nasopharyngeal carcinoma |
| Experimental category: | L |
| PMID: | 30535454 |
| Experiment: | CCK-8 assay//Colony formation assay//SA-β-gal activity assay//Flow cytometry |
| Description: | The effect of CD38 on the proliferation of 5-8F cells was examined using a CCK-8 assay. The results demonstrated that CD38 promoted the proliferation of 5-8F cells .Acolony formation assay was performed. The results indicated that the high expression of CD38 clone number is increased, compared with the control group; The results demonstrated that the number of β-galactosidase-positive 5-8F/CD38 cells was reduced, compared with 5-8F/Vector cells. Additionally, it was demonstrated that CD38 overexpression reduced cellular senescence in NPC cells.This demonstrated that CD38 promoted S phase DNA replication and promoted the proliferation of NPC cells. |
| Target gene: | CDK4//MITOGEN-ACTIVATEDPROTEINKINASE9//CYCLIN D1 |
| Official symbol(s): | CDK4//MITOGEN-ACTIVATEDPROTEINKINASE9//CYCLIN D1 |
| R-AG-Target gene: | Upregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Upregulation of cell proliferation-associated genes CDK4, mitogen-activated protein kinase 9 and Cyclin D1 progressed the cell cycle from the G1 phase to the S phase resulting in increased cell proliferation. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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