| Gene name: | EGLN1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HHUA |
| Gene ID: | 54583 |
| Category: | protein coding |
| Phenotype: | Endometrial cancer |
| Experimental category: | L |
| PMID: | 16161047 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay |
| Description: | Seven clones out of HHUA transfectants, 8 of Ishikawa and 9 of HWCA showed flattened cytoplasm, frequently accompanied with multinuclei formation analogous to replicative cell senescence. A significantly increased fraction of acid-β-gal positive cells in the transected clones indicated the induction of cancer cell senescence by exogenous EGLN1 expression. |
| Target gene: | P21//HTERT//HIF-1α |
| Official symbol(s): | P21//HTERT//HIF1A |
| R-AG-Target gene: | Upregulation//Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | In contrast, only the p21 CDK inhibitor was significantly upregulated in response to the EGLN1 transfection.Downregulation of hTERT was also observed in passage 5 of HHUA clone 3-2 and 122.As expected, both HHUA and Ishikawa cells silenced HIF-a expression in response to EGLN1 expression, followed by the suppressed expression of VEGF, which is a downstream transcriptional target of HIF-1a. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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