| Gene name: | MAP2K3 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 184A1,MCF 10A |
| Gene ID: | 5606 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 21137025 |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | Overexpression of MAP2K3 in 184A1 and MCF10A cells resulted in pronounced senescence of cells.PD rate of MAP2K3-expressing cells was decreased already after 3 days, and was halted by day 6, while the control cells continued to proliferate. When SA-β-galactosidase was measured, we observed that expression of MAP2K3 strongly increased its activity. Measurement of cell proliferation showed that expression of MAP2K3 had an inhibitory effect. |
| Target gene: | P53//P38//PRB |
| Official symbol(s): | P53//P38//PRB |
| R-AG-Target gene: | Upregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We measured the levels of p53, p38, pRB and Erk1/2 in cells transfected with MAP2K3 expression vector. We observed that enhanced expression of MAP2K3 led to increase of p53, pRB and p38 expression, but not Erk1/2. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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