| Gene name: | HMGB1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Embryo |
| Cell name: | IMR-90,HCA2,HMEC,MEF |
| Experiment: | Western blot//SA-β-gal activity assay//Knockdown |
| Description: | We depleted HMGB1 in human fibroblasts (IMR-90, HCA2) that reduced intracellular HMGB1 protein levels by >90%. HMGB1-depleted cells underwent senescence, as judged by arrested growth, senescence-associated heterochromatin foci (SAHF) formation (Narita et al., 2003), and SA-β-Gal activity.HMGB1 depletion also caused senescence in human mammary epithelial cells (HMECs) and MEFs, indicating the response was not restricted to human fibroblasts. |
| Target gene: | P53//NF-κB |
| Official symbol(s): | P53//NFKB1 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Western blot//ELISA assay |
| Target gene description: | Consistent with the IL-6 ELISA results, NF-κB activity was robust in HMGB1-overexpressing cells, but not HMGB1-depleted cells,senescence caused by HMGB1 overexpression or depletion was p53 dependent, as determined by SA-β-Gal activity, growth arrest (not depicted), and SAHF formation. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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