| Gene name: | PML |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | SA-β-gal activity assay//BrdU assay |
| Description: | Cells arrested proliferation shortly after selection and remained arrested with features of cellular senescence, that is, a flat cell morphology and positive staining for the senescenceassociated β-galactosidase;PML induced a growth arrest with the characteristics of cellular senescence. |
| Regulatory pathway: | RB |
| R-AG-Pathway: | -- |
| Official symbol(s): | retinoblastoma |
| Pathway experiment: | SA-β-gal activity assay//3H-thymidine incorporation assay |
| Pathway description: | Cells expressing this mutant entered senescence in response to PML with higher efficiency than wild-type HDFs, according to a3 H-thymidine incorporation assay that measures cell cycle arrest, and according to the SA-β-gal assay that measures senescence,Therefore, the ability of E7 to by pass PML-induced senescence requires inactivation of the Rb tumor-suppressor pathway. |
Annotation:
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