| Gene name: | TPR |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HeLa |
| Experiment: | Knockdown//FACS analysis//Colony formation assay//SA-β-gal activity assay |
| Description: | Treatment with Tpr siRNAs impaired cell proliferation as compared to mock siRNA-treated cells.The cell cycle was arrested at the G0–G1 phase in Tpr knockdown cells A substantial decrease (36%) in the number of colonies was apparent when cells were transfected with Tpr siRNAs.most of the enlarged and flattened cells were β-gal positive and colored blue (about 40%) in Tpr knockdown wells. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Knockdown//Western blot |
| Pathway description: | Levels of p53 and p21 were both up-regulated in Tpr-depleted Hela cells and U2OS cells .Knocking down both Tpr and p53 resulted in a substantial reversion of the senescence phenotype as assessed by the percentage of SA-β-galactosidase-positive cells. |
Annotation:
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