| Gene name: | SPOP |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Gene ID: | 8405 |
| Category: | protein coding |
| Phenotype: | Prostate cancer |
| Experimental category: | L |
| PMID: | 26527005 |
| Experiment: | SA-β-gal activity assay//Western blot//BrdU assay//Knockdown |
| Description: | Ectopic SPOP expression induced expression of markers of senescence such as SA-β-gal activity and upregulation of p53 and p21, while it suppressed cell proliferation markers .SPOP knockdown suppressed the expression of senescence markers such as SA-β-gal activity, SAHF formation and upregulation of p53 and p21.cell proliferation markers were significantly increased in shSPOP/RAS cells compared with RAS-expressing cells. |
| Target gene: | SENP7//HP1α |
| Official symbol(s): | SENP7//CBX5 |
| R-AG-Target gene: | Downregulation//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Co-IP//Immunohistochemistry//Knockdown |
| Target gene description: | Ectopic SPOP and SENP7 co-immunoprecipitated with each other;here was a SPOP dose-dependent decrease in SENP7 expression , suggesting that SPOP promotes SENP7 degradation.; SENP7 is expressed at significantly (p<0.0001) higher levels in prostate tumors with SPOP mutations compared to those with wild-type SPOP.SPOP knockdown caused a decrease in the levels of sumoylated HP1α in senescent cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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