| Gene name: | YAP1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PDLSC |
| Experiment: | Knockdown//EdU assay//Apoptosis assay//SA-β-gal activity assay//Flow cytometry |
| Description: | EdU result showed that the proliferation rate of shYAP reduced markedly after transfection compared with NC group (P<0.01)；The percentage of early and late apoptotic cells in shYAP group were significantly increased after YAP knockdown compared with NC group (p<0.001)；compared with NC group, the proportion of cells in G0/G1 phase increased evidently (P < 0.05), while that in S and G2/M phase decreased (P < 0.05) in shYAP group；shYAP group showed higher senescence cells rate compared with NC group (P<0.01). |
| Regulatory pathway: | ERK//BCL-2 |
| R-AG-Pathway: | Upregulation//Downregulation |
| Official symbol(s): | MAPK1//BCL2 |
| Pathway experiment: | Knockdown//Western blot |
| Pathway description: | The expression of p-C-Raf338 and p-MEK, which take part in the phosphorylation of Erk, was inhibited when YAP was knocked down; Bcl-2 family members (Bak, Bax, Bad, Bid and Bik), their expression levels increased separately after knocking down YAP. |
Annotation:
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