| Gene name: | STMN1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Immunofluorescence//Knockdown |
| Description: | Microscopic observation of the stathmin-depleted IMR-90 cells revealed enlarged and flattened cells, suggesting that they might be undergoing senescence. Compared to the control-shRNA treated cells, stathmin-shRNA treated and hydrogen peroxide-treated cells showed blue staining with SA-β-gal. Over 80% percent of stathmin-depleted IMR-90 cells stained positive, whereas only 6% of hydrogen peroxide-treated cells were stained .Levels of Ser 15 phosphorylated as well as total p53 were much higher in stathmin-depleted IMR-90 cells when compared with the control shRNA-treated cells . Phosphorylated Rb at Ser 780 was barely detected in stathmin-depleted IMR-90 cells, while it was easily detected in control shRNA-treated cells. The amount of total Rb was similar in positive control, control and stathmin shRNA-treated cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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