| Gene name: | POU5F1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PA-1 |
| Gene ID: | 5460 |
| Category: | protein coding |
| Phenotype: | Embryonal carcinoma |
| Experimental category: | L |
| PMID: | 26102294 |
| Experiment: | Flow cytometry//SA-β-gal activity assay//Cell morphological analysis |
| Description: | OCT4A-silenced cells underwent a profound G2M arrest with apoptosis being greatly reduced. The cells displayed the large, flattened morphology and Sa-β-gal staining associated with senescence on day 5, identical to that previously reported when silencing p53. Prolonged loss of OCT4A lead to the polyploidisation of these senescent cells and a reduction in clonal recovery. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown |
| Target gene description: | Efficient (80–95%) knock-down of OCT4A was confirmed with 3 different siRNA, in addition to a 3 siRNA pool and the molecular response to ETO was then examined by immunoblotting for key regulators of DNA damage, (pCHK2, p53, p21Cip1, and RAD51).These experiments revealed p53, RAD51 and pCHK2 were all upregulated from day 1 independently of OCT4A expression. p21Cip1 expression rose gradually from day 1 throughout the course of experiment as seen in previous experiments. Silencing of OCT4A resulted in a marked increase in p21Cip1 expression from day 1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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