Gene name: | IFNB1 |
Aging type: | Accelerate |
Aging characteristic: |
Tissue type: | -- |
Cell name: | LXSN,K16,K38,ME-180,Caski,HeLa,SiHa |
Experiment: | SA-β-gal activity assay |
Description: | Increasingly high percentages of senescent cells were observed exclusively in K38 cells transformed by E6 and E7 proteins of cutaneous HPV genotype, starting from 4 days of IFN-β treatment, compared to control keratinocytes (LXSN), K16 cells and mucosal high risk HPV-positive cell lines. |
Target gene: | PML//P21//P53 |
Official symbol(s): | PML//P21//P53 |
R-AG-Target gene: | Upregulation//Upregulation//Activation |
Subcategory: | Acetylation Phosphorylation |
Target gene experiment: | Western blot//qRT-PCR |
Target gene description: | The expression of HPV38 E6 and E7 in human keratinocytes induces the stabilization of p53, as shown by WB analysis of p53 in K38 cells compared with control keratinocytes (LXSN), K16 cells and high risk HPV-positive cell lines SiHA and ME-180. Three different siRNAs were used for PML, p53 and p21 genes. Upon IFN-β treatment, PML was up-regulated as well as p21. PML seems to be an essential component of senescence response in K38 cells, since, when PML expression is inhibited by specific siRNAs, IFN-β-induced senescence is strongly reduced. p21 silencing partially affects IFN-β-induced senescence, while p53 silencing appears much more effective, IFN-β modulates p53 phosphorylation status at different phosphorylation sites while acetylation is mainly downregulated in Lys-320.In fact, when PML expression is silenced, DNp73 protein levels are not downregulated by IFN-β. The DNp73 protein expression appears to be reduced upon IFN-β treatment, probably as a result of the p53 post-translational modifications induced by IFN-β.In fact, when PML expression is silenced, DNp73 protein levels are not downregulated by IFN-β.PML depletion reduces IFN-β induction of Bax and Pig3 in K-38 cells, indicating the role of PML in the ability of IFN-β to recover p53 transactivation activity of specific target genes. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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