Gene name: | NR0B2 |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | CNE-1,CNE-2 |
Gene ID: | 8431 |
Category: | protein coding |
Phenotype: | Nasopharyngeal carcinoma |
Experimental category: | L |
PMID: | 26215037 |
Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
Description: | Morphologically, CNE-2 and CNE-2-empty vector cells were vacuolated, flattened and much larger in size compared with CNE-2 SHP-1 overexpression cells.β-galactosidase staining revealed higher senescence in CNE-1 SHP-1 shRNA cells compared with CNE-1-scramble shRNA cells (23.6?±?3.4 % vs. 11.4?±?1.8 %, P?0.001), and lower senescence in CNE-2 SHP-1 overexpression cells compared with CNE-2-empty vector cells (3.6?±?2.7 % vs. 13.2?±?3.3 %, P?=?0.001.Compared with CNE-2-empty vector cells, CNE-2 SHP-1 overexpression cells had lower proportions of cells in G1 (55.7?±?2.6 % vs. 71.8?±?2.9 %, P?0.001) and G2/M (4.7?±?0.8 % vs. 8.18?±?1.3 %, P 0.001) phases, and a higher proportion of cells in S phase (39.7?±?2.2 % vs. 20.1?±?2.9 %, P?=?0.001). |
Target gene: | P16//PRB |
Official symbol(s): | P16//PRB |
R-AG-Target gene: | Downregulation//Upregulation |
Subcategory: | Unclear |
Target gene experiment: | Western blot |
Target gene description: | On the other hand, compared with CNE-2-empty vector cells, CNE-2 SHP-1 overexpression cells showed decreased expression of p16 (?95 %, P?0.001), and increased expressions of Rb (+358 %, P?0.001) and pRb (+248 %, P?0.001). |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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