| Gene name: | CD40LG |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A549,H460 |
| Gene ID: | 959 |
| Category: | protein coding |
| Phenotype: | Lung cancer |
| Experimental category: | L |
| PMID: | 30078020 |
| Experiment: | SA-β-gal activity assay//EdU assay |
| Description: | Results showed a greater number of positively stained CD40L-WT/CD40L-M cells than control cells in A549/TR, A549/DDP, and H460 cells.We then explored the effects of CD40L-WT/CD40L-M overexpression on DNA synthesis and cell proliferation, which are characteristics of cell senescence. The results showed that overexpression of CD40L-WT/CD40L-M inhibited DNA synthesis and cell proliferation. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | -- |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Immunostaining//SA-β-gal activity assay//Western blot |
| Pathway description: | CD40L-WT or CD40L-M-overexpressed cells showed nuclear translocation of NF-κB p65.To address the functional significanof NF-κB in the senescence program of CD40L-M-overexpressing NSCLC cells, transfected cells were treated with the NF-κB inhibitor Bay11-7082 . Results of this experiment showed a decrease in the expression of p53. Further, NF-κB signaling was inhibited via p65 siRNA, and the protein levels of p53 and p21 were attenuated. SA-β-gal staining was also detected. |
Annotation:
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