| Gene name: | CARF |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Fibroblast |
| Experiment: | SA-β-gal activity assay//Autofluorescence |
| Description: | We examined senescence specific markers in control and CARF-overexpressing cells and found that consistent with senescence-like morphology and loss of proliferation, the CARF-overexpressing cells showed autofluorescence, senescence-specific -gal staining and enhanced levels of p53 protein and its downstream effector, p21WAF1. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | SA-β-gal activity assay//Western blot |
| Pathway description: | We have found that CARF is up-regulated in senescent fibroblasts and showed a strong correlation with β-gal activity as well as increase in p53 activity and p21WAF1 levels, established molecular markers of senescence . |
Annotation:
Loading,please wait...