| Gene name: | DIP2C |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | RKO |
| Gene ID: | 22982 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 28716088 |
| Experiment: | SA-β-gal activity assay//RT-qPCR//Knockdown |
| Description: | We performed qPCR and found p14 ARF and p16 INK4a upregulated to the same extent in DIP2C ?/? cells . As p16 INK4a is a marker of cellular senescence we then stained cells for β-galactosidase activity at pH 6, which is another characteristic of senescent cells, detecting staining of up to 1.9% of DIP2C ?/? cells, compared to 0.2% of parental RKO cells.Cell enlargement is another sign of senescence and imaging of live cells revealed that DIP2C knock-out cells appeared stretched-out relative to parental RKO cells . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...