| Gene name: | TNF |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HMVEC |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Staining with SA-β-gal, pH 6.0 revealed a significant increase (N52%) of SA-β-gal positive cells in young HMVEC-Ls treated with 10 and 20 ng/ml TNF-α for 15 consecutive treatments over a period of 15 days when compared to the young, untreated cells. In parallel with an increase in the SA-β-gal expression, young HMVEC-Ls exposed to chronic TNF-α treatment became enlarged and flattened, which is a typical senescent phenotypic morphology, similar to those of the RS cells.There were 5.5 ± 1.7% SAβ-gal positive in untreated young cells whereas both RS and chronic TNF-α-treated young untreated cells demonstrated significantly higher SA-β-gal positive cells, which were ~49.4 ± 4.6% and 57.8 ± 10.0%, respectively.The TNF-α-treated cell and RS cell cultures contained significantly higher percentages of G0/G1 cell population i.e. ~67% and ~71%, respectively, compared to the untreated young cell culture (~54%). |
| Target gene: | HSA-MIR-20B |
| Official symbol(s): | HSA-MIR-20B |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | qRT-PCR results corroborated with microarray expression, whereby hsa-miR-17 and -20a were downregulated in RS and TNF-α-treated cells when compared to young untreated cells .However, hsa-miR-20b was instead found upregulated with qRT-PCR analysis and this discrepancy could be due to the inherent differences between the two methods in detecting miRNAs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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