| Gene name: | PRC2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | TIG-3 |
| Experiment: | SA-β-gal activity assay//Western blot//qPCR//CHIP |
| Description: | Therefore, to formally establish that a decreasing pool of the PRC2 complex directly leads to senescence and displacement of BMI1, we targeted the expression of EZH2 and SUZ12 by short hairpin interference.The loss of either protein results in the disruption of the PRC2 complex (Pasini et al. 2004b) and leads to the decrease of H3K27me3 and displacement of both BMI1 and CBX8 from the INK4A promoter. Significantly, cells depleted of EZH2 or SUZ12, similar to cells depleted of BMI1, activate INK4A transcription and become senescent . |
| Target gene: | INK4A |
| Official symbol(s): | INK4A |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//SA-β-gal activity assay |
| Target gene description: | We targeted the expression of EZH2 and SUZ12 by short hairpin interference. The loss of either protein results in the disruption of the PRC2 complex (Pasini et al. 2004b).cells depleted of EZH2 or SUZ12, similar to cells depleted of BMI1, activate INK4A transcription and become senescent. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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