| Gene name: | SYK |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A375 |
| Experiment: | SA-β-gal activity assay//Immunofluorescence//Cell morphological analysis |
| Description: | We observed that a major population of cells arrested by Syk displayed a flat, enlarged morphology with increased granularity that is characteristic of senescent cells .A blue staining indicative of acidic β-gal activity was observed in the growth-arrested A375 infected with Ad-Syk. Notably, ～40% of cells were β-gal positive in Syk-expressing populations, whereas <2% of A375 cells were positive in vector-infected cultures. Immunofluorescence analysis for HP1β, a marker of SAHF, revealed punctuated regions of DNA corresponding to heterochromatic foci in Syk-expressing cells, but not in control Syk-negative cells. |
| Regulatory pathway: | P53-P21//PRB |
| R-AG-Pathway: | Upregulation//Upregulation |
| Official symbol(s): | TP53-CDKN1A//retinoblastoma |
| Pathway experiment: | Western blot//immunofluorescence |
| Pathway description: | Increased levels of activating phosphorylation of p53 at Ser15 and induction of the cdk inhibitor p21 were observed in Syk-expressing A375 cells compared with controls . We also noticed that Syk expression resulted in modest, but consistent, increase in the levels of total p53. In addition, Syk-expressing cells harbored dephosphorylation of pRb and lost cyclin A expression. Consistent with changes in p53 regulation, immunofluorescence of individual cells showed nuclear p53 accumulation in Syk-positive cells . |
Annotation:
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