| Gene name: | HDAC1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | UCD-Mel-J |
| Experiment: | Western blot//MTT assay//SA-β-gal activity assay |
| Description: | The Tet-regulated UCD-derivative (UCD-HDAC1) cell line expressed HDAC1 when treated with doxycycline (Dox), a potent tetracycline analog. An MTT-cell proliferation assay demonstrated that 10–14 days after HDAC1 induction, UCD-HDAC1 cells became growth arrested,whereas uninduced UCD-HDAC1 cells, or UCD cells harboring an empty vector in the presence or absence of Dox, displayed normal exponential growth.After 3 weeks of sustained HDAC1 levels the cells became irreversibly arrested.These cells did not resume growth upon Dox removal, and continued to display typical markers of normal melanocyte senescence (Bennett & Medrano, 2002) including expression of the senescent-associated β-galactosidase (SA-β-gal) marker, a flat morphology, and aberrant cytokinesis. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...