Gene name: | PPARD |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | VSMC |
Experiment: | SA-β-gal activity assay//Western blot |
Description: | Activation of PPARδ by GW501516, a specific ligand for PPARδ, significantly attenuated Ang II-induced generation of superoxides and suppressed senescence of VSMCs.When VSMCs were treated with Ang II, the senescence-associated β-galactosidase (SA β-gal) activity, a biomarker for cellular senescence, was significantly increased in Ang II-treated VSMCs.This increase was significantly suppressed in the presence of GW501516, suggesting the involvement of PPARδ in the inhibition of Ang II-induced premature senescence . The siRNA-mediated down-regulation of PPARδ significantly suppressed the GW501516-mediated inhibition of premature senescence of VSMCs induced by Ang II. Similar results were obtained when the cells were analyzed with another PPARδ siRNA_2. |
Target gene: | PTEN |
Official symbol(s): | PTEN |
R-AG-Target gene: | Upregulation |
Subcategory: | Unclear |
Target gene experiment: | SA-β-gal activity assay//Western blot |
Target gene description: | Treatment with GW501516 significantly increased the level of PTEN transcript in a time-dependent manner. The up-regulation of PTEN by GW501516 was suppressed in the presence of siRNA against PPARδ, suggesting the causal role of PPARδ in the up-regulation of PTEN.PTEN siRNA reversed the GW501516-mediated suppression of superoxide generation in Ang II-treated cells. The number of SA β-gal-positive cells reduced by GW501516 was also recovered by transfection with PTEN siRNA. |
Regulatory pathway: | PI3K-AKT |
R-AG-Pathway: | -- |
Official symbol(s): | PIK3CA-AKT1 |
Pathway experiment: | Western blot |
Pathway description: | The activation of Akt by Ang II was markedly reduced in the presence of GW501516 and almost completely abolished in the presence of both GW501516 and LY294002. Down-regulation of PPARδ by siRNA reversed the GW501516-induced decrease in phosphorylated Akt in cells treated with Ang II. Similar results were obtained with another PPARδ siRNA_2.The short time pretreatment (30 min) did not affect the level of phosphorylated Akt, whereas the long time pretreatment (24 h) significantly suppressed the Ang II-induced increase in phosphorylated Akt. |
Annotation:
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