Aging Overview

Aging gene

Gene name: PPARD
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: VSMC
Gene ID: 5467
Category: protein coding
Phenotype: Aging
Experimental category: L
PMID: 22072715
Experiment: SA-β-gal activity assay//Western blot
Description: Activation of PPARδ by GW501516, a specific ligand for PPARδ, significantly attenuated Ang II-induced generation of superoxides and suppressed senescence of VSMCs.When VSMCs were treated with Ang II, the senescence-associated β-galactosidase (SA β-gal) activity, a biomarker for cellular senescence, was significantly increased in Ang II-treated VSMCs.This increase was significantly suppressed in the presence of GW501516, suggesting the involvement of PPARδ in the inhibition of Ang II-induced premature senescence . The siRNA-mediated down-regulation of PPARδ significantly suppressed the GW501516-mediated inhibition of premature senescence of VSMCs induced by Ang II. Similar results were obtained when the cells were analyzed with another PPARδ siRNA_2.


Regulatory relationship

Target gene: PTEN
Official symbol(s): PTEN
R-AG-Target gene: Upregulation
Subcategory: Unclear
Target gene experiment: SA-β-gal activity assay//Western blot
Target gene description: Treatment with GW501516 significantly increased the level of PTEN transcript in a time-dependent manner. The up-regulation of PTEN by GW501516 was suppressed in the presence of siRNA against PPARδ, suggesting the causal role of PPARδ in the up-regulation of PTEN.PTEN siRNA reversed the GW501516-mediated suppression of superoxide generation in Ang II-treated cells. The number of SA β-gal-positive cells reduced by GW501516 was also recovered by transfection with PTEN siRNA.

Regulatory pathway: PI3K-AKT
R-AG-Pathway: --
Official symbol(s): PIK3CA-AKT1
Pathway experiment: Western blot
Pathway description: The activation of Akt by Ang II was markedly reduced in the presence of GW501516 and almost completely abolished in the presence of both GW501516 and LY294002. Down-regulation of PPARδ by siRNA reversed the GW501516-induced decrease in phosphorylated Akt in cells treated with Ang II. Similar results were obtained with another PPARδ siRNA_2.The short time pretreatment (30 min) did not affect the level of phosphorylated Akt, whereas the long time pretreatment (24 h) significantly suppressed the Ang II-induced increase in phosphorylated Akt.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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