| Gene name: | HDAC9 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Experiment: | SA-β-gal activity assay//EdU assay//Western blot |
| Description: | We show that sodium butyrate (NaB) and panobinostat (LBH589), two broad-spectrum HDAC inhibitors up-regulate hsa-miR-31 (miR-31).The results of the Western blot analysis showed that NaB and LBH589 induced p53, p21, and p16, and reduced comparative levels of phosphorylated pRb in control cells.The results indicated that NaB and LBH589 induced a robust senescent phenotype in control cells .The results indicated that NaB and LBH589 increased γH2AX foci formation in control cells. |
| Target gene: | MIR-31 |
| Official symbol(s): | MIR-31 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//EdU assay//Western blot//γH2AX staining |
| Target gene description: | The results of the Western blot analysis showed that NaB and LBH589 induced p53, p21, and p16, and reduced comparative levels of phosphorylated pRb in control but not in miR-31 inhibitor expressing cells. The data also indicated that the miR-31 inhibitor overcomes the proliferation inhibitory effect of NaB and LBH589 in MRC5 cells, and that the miR-31 inhibitor promotes cell proliferation. The results indicated that NaB and LBH589 induced a robust senescent phenotype in control but not in miR-31 inhibitor expressing cells as indicated by an increase in SA-β-gal- and decrease in EdU-positive cells.The results indicated that NaB and LBH589 increased γH2AX foci formation in control but not in miR-31 inhibitor expressing cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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