| Gene name: | ABI3BP |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ARO |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | We first observed that SA-β-Gal positive cells,indicated by blue staining, were increased when ABI3BP is re-expressed.Secondly, immunofluorescence of HP1γ showed an expression pattern concentrated in DNA foci of cells expressing ABI3BP compared to controls which suggested that the re-expression of ABI3BP was associated with the nuclear changes that occur during senescence. Conversely in vector-transfected cells, the staining for HP1γ gave a weak signal and it was disperse through the nucleoplasm .Cell cycle distribution was analyzed by flow cytometry. G0/G1 arrest occurred in ARO cells expressing ABI3BP as indicated by an increase in the percentage number of cells at this phase. |
| Regulatory pathway: | P21 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | CDKN1A |
| Pathway experiment: | qPCR |
| Pathway description: | P21 mRNA levels were 3-fold increased in ARO cells following ABI3BP re-expression . G0/G1 arrest occurred in ARO cells expressing ABI3BP as indicated by an increase in the percentage number of cells at this phase. Although cell cycle was not investigated at day 5, when senescence phenotype was marked observed, the cell proliferation marker Ki67 was down-regulated and the cell cycle inhibitor p21 was up-regulated at day 5. |
Annotation:
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