| Gene name: | HSP27 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Experiment: | SA-β-gal activity assay//qPCR//Knockdown |
| Description: | After 3 days of HSP27 knockdown, we observed significant increases in cells positive for senescence-associated β-gal (SA-β-gal) and significant up-regulation of pro-inflammatory cytokines IL-1α and IL-8. |
| Target gene: | E2F4//P130 |
| Official symbol(s): | E2F4//P130 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | qPCR//Western blot//Immunofluorescence |
| Target gene description: | By using qPCR, we found that the expression of E2F-4 was significantly increased upon HSP27 knockdown, HSP27 knockdown increased the expression of p130.We also confirmed strong increases of E2F-4 and p130 proteins by HSP27 knockdown using immunoblot analysis and immunocytochemistry. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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