| Gene name: | DPY30 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | Colony formation assay//SA-β-gal activity assay//SAHF//Cell morphological analysis//Knockdown |
| Description: | ShDPY30 IMR90 cells also contained DAPI-stained SAHFs, another characteristic for senescent human fibroblasts (Narita et al, 2003). Additionally, the overall morphology of the DPY30 knockdown IMR90 cells changed, towards a more rounded and flattened phenotype with enlarged nuclei. SA-β-galactosidase assays showed that >90% of the shDPY30 IMR90 cells expressed Activate SA-β-galactosidase. Further, in proliferation assays, growth curves, and colony formation assays, shDPY30 IMR90 cells arrested and stopped proliferating. |
| Target gene: | ID |
| Official symbol(s): | ID1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//SA-β-gal activity assay |
| Target gene description: | In ID1 and ID3 overexpressing cells, DPY30 knockdown reduced mRNA levels, but not proteins levels, of ID1 and ID3.the shDPY30 cells with ID1 or ID3 overexpression regained their fibroblast features and began to proliferate again, albeit not to the same degree as the IMR90 control cells, while the shDPY30 cells remained in a senescence-like state.?A reduced SA-β-galactosidase staining was observed in shDPY30 cells overexpressing either ID1 or ID3 (from 82 to 55 or 28%, respectively). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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