| Gene name: | DAO |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U2OS,HepG2 |
| Experiment: | SA-β-gal activity assay//Colony formation assay//Western blot//EdU assay |
| Description: | We observed that the percentage of SA-β-gal–positive cells was increased after the treatment with etoposide both in U2OS and HepG2 cells, knockdown of DAO partially restored etoposide-induced loss of proliferative capacity.we next tested the impact of inhibiting DAO activity on senescence using 6-chlorobenzo[d]isoxazol-3-ol (CBIO), previously characterized for the ability to inhibit enzymatic activity of DAO .CBIO suppressed the SA-β-gal activation and loss of proliferative capacity following etoposide treatment in both U2OS and HepG2 cells.CBIO was also effective in suppressing senescence of Hs68 cells, as judged by SA-β-gal, EdU incorporation proliferation assays, and immunoblot analysis, We observed that ectopic expression of DAO enhanced etoposide-induced senescence, although DAO overexpression in the absence of etoposide had no significant effect, indicating that DAO expression alone is not sufficient to induce senescence but accelerates senescence under stress conditions. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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