| Gene name: | GADD45G |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SK-Hep-1,Hep3B,SMMC-7721 |
| Gene ID: | 10912 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 23897841 |
| Experiment: | SA-β-gal activity assay//RT-PCR |
| Description: | Approximately 50%‐70% of these cells ectopically expressing GADD45G were stained positively for senescence‐associated marker β‐galactosidase . Furthermore, mRNA levels of the senescence‐associated secretory cytokine, interleukin (IL)‐8, were also significantly elevated. |
| Regulatory pathway: | JAK-STAT3 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | hop-STAT3 |
| Pathway experiment: | Western blot//SA-β-gal activity assay |
| Pathway description: | We found that the constitutive phosphorylation of Stat3 (Tyr705) was substantially inhibited by GADD45G expression in the human liver cancer cell lines, Sk‐Hep1, SMMC‐7721, and Hep3B.In Sk‐Hep1 cells, we found that levels of Tyr705‐phosphorylated Stat3 rapidly decreased, starting within 2 hours after GADD45G induction; coincidently, levels of phosphorylated Jak2 and tyrosine kinase 2 (Tyk2), the upstream activators of Stat3, were also severely down‐regulated.We extended our observation to two subclones (numbers 1 and 2) of mouse LPC‐H‐RasV12 cells, wherein GADD45G could significantly induce cell senescence.Western blot levels of phosphorylated Stat3, Jak2, and Tyk2 were also substantially decreased in these cells . |
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