| Gene name: | DGCR8 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Gene ID: | 54487 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 23773483 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | shDGCR8 IMR90 cells also showed a significant increase in the number of cells with senescent‐associated beta‐galactosidase (SA‐Beta Gal) activity as well as nuclei with SAHFs (senescence‐associated heterochromatin foci), regions of facultative heterochromatin characteristic of senescent cells. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | BrdU assay |
| Target gene description: | Inactivation of DGCR8 in human and murine fibroblasts caused a modest, but reproducible increase in the protein levels of the cell‐cycle inhibitor p21CIP1. Also, a 3‐fold increase in p21CIP1 transcript and increased number of p21CIP1‐positive cells by immunofluorescence was observed in shDGCR8 human fibroblasts. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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