| Gene name: | THRB |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ARO,MCF-7,COS-7 |
| Experiment: | SA-β-gal activity assay |
| Description: | We examined SA-β-gal activity and found that the number of positively stained cells was significantly higher in the irradiated cultures overexpressing wtTHRB than in those treated by any singular agent or by any other combination. Effect of wtTHRB-Ad on the accumulation of SA-β-gal-positive cells was more than additive in irradiated MCF-7 and COS7 cultures, and additive in ARO, Infection with mutTHRB-Ad significantly decreased the number of SA-β-gal-positive cells in irradiated ARO and COS7 cultures. |
| Target gene: | P21//P16//RB |
| Official symbol(s): | P21//P16//RB |
| R-AG-Target gene: | Upregulation//Upregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | One hundred-twenty hours after irradiation, i.e. when growth inhibition and senescence-like changes have already taken place in the cultures, cultures infected with wtTHRBAd consistently displayed the decreased phosphorylation of Rb, suggestive of cell accumulation in the G1 phase. Concordantly, these cells also had elevated p21. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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