| Gene name: | ZEB1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SW480 |
| Gene ID: | 6935 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 27965283 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | We found that ZEB1 knockdown increased the number of SA β-gal-positive cells and that Wnt3a inhibited basal SA β-gal activity in SW480-shCtl cells but not in SW480-shZEB1-A cells. |
| Target gene: | DDK1//MUTANT P53//MDM2//CTBP//MACROH2A1 |
| Official symbol(s): | MGME1//MUTANT P53//MDM2//CTBP//MACROH2A1 |
| R-AG-Target gene: | Activation//Activation//Activation//Activation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot//CHIP//qRT-PCR |
| Target gene description: | Accordingly, the transcriptional activity of serial deletions of the DKK1 promoter increased when the site at?490 bp was included.The ability of endogenous ZEB1 to directly bind to the DKK1 promoter was tested for the site at ?490 bp by ChIP assay.Furthermore, mutation of the CtBP binding sites in ZEB1 (ZEB1-CIDmut) hampered ZEB1’s repression of H2AFY,We found that blocking of CtBP activity in SW480 cells by MTOB induced senescence and upregulated H2AFY.We found that overexpression of DKK1 or addition of rhDKK1, but not rhDKK3, in mutant SW480 cells upregulated mutant TP53 and MDM2 expression in these cells . In turn, DKK1 knockdown downregulated both mutant TP53 and MDM2 and this downregulation was reverted by addition of rhDKK1, but not of rhDKK3. The downstream effect of DKK1 via mutant p53 was corroborated by the knockdown of the latter with a specific siRNA (siTP53). siTP53, but not siCtl, downregulated mRNA expression levels of MDM2 and CTBP while increasing those of H2AFY . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...