| Gene name: | TBX2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Gene ID: | 6909 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 20348948 |
| Experiment: | Clonogenic assay//Knockdown//SA-β-gal activity assay |
| Description: | We performed TBX2 siRNA knockdown in three breast cancer cell lines and after confirming knockdown we assessed effects on cell growth using clonogenic assays. We observed dramatic growth inhibition in all three cell lines.Finally, using senescence-associated β-galactosidase staining we could also show that knockdown of either TBX2 or EGR1 by transfection of NDRG1 into MCF7 cells that resulted in increased expression of Dec1, indicating that NDRG1 may contribute to the cell senescence observed after TBX2 inhibition. |
| Target gene: | NDRG1 |
| Official symbol(s): | NDRG1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | Western blot//RT-PCR//Knockdown |
| Target gene description: | The most consistently TBX2-regulated gene was NDRG1, which showed consistent upregulation after TBX2 siRNA in several breast cancer cell lines. We also observed NDRG1 upregulation both at the mRNA and protein level after induction of DN-TBX2 in MCF7 cells . Together, these data show that TBX2 represses multiple target genes with NDRG1 consistently upregulated after TBX2 inhibition. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...