| Gene name: | ATM |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Furthermore, knockdown of ATM by siRNA suppressed increase in SA-β-gal-positive cells induced by H2O2. |
| Regulatory pathway: | ATM-AKT-P53-P21 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | ATM-AKT1-TP53-CDKN1A |
| Pathway experiment: | Western blot//RT-PCR//SA-β-gal activity assay |
| Pathway description: | To assess whether ATM phosphorylation was caused by H2O2-induced redox imbalance. Western blot analysis showed that pretreatment of endothelial cells with NAC blocked the stimulatory effects of H2O2 on the expression of ATM-S1981, Akt-S473, p53-S15 and p21 .Cells transfected with ATM siRNA showed reduced ATM expression with inhibition of Akt and p53 phosphorylation in addition to down-regulation of p21 expression .Moreover, down-regulation of Akt, p53, or p21 by siRNA also suppressed an increase in SA-β-gal-positive cells induced by H2O2. |
Annotation:
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