| Gene name: | BMI1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | GIST882 |
| Gene ID: | 648 |
| Category: | protein coding |
| Phenotype: | Gastrointestinal stromal tumor |
| Experimental category: | L |
| PMID: | 29042141 |
| Experiment: | SA-β-gal activity assay//Knockdown//Colony formation assay//CCK-8 assay |
| Description: | The β-Gal activity assay was applied in order to detect cell senescence . In comparison with the control group, the percentage of senescent cells involved with the Bmi-1 shRNA group significantly increased (26.7% ± 3.9% vs. 17.2% ± 2.7%; P < 0.05).The results of the soft agar colony formation assay indicated that the number of colonies contained in the control group was closer to that in the NC group (9.5 ± 3.1 vs. 10.1 ± 2.2; P > 0.05). In comparison with the control group, the number of colonies in the Bmi-1 shRNA group were reduced significantly (9.5 ± 3.1 vs. 3.2 ± 1.5; P < 0.05). |
| Target gene: | P16//P14 |
| Official symbol(s): | P16//P14 |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot//Knockdown |
| Target gene description: | The results of the RT-qPCR method for detection of the mRNA expressions of Bmi-1, p16Ink4A, p14ARF, cyclinD 1, and CDK4 in the GIST882 cells among the three groups are shown . These results showed no remarkable differences of Bmi-1 mRNA expression between the control and NC groups (P > 0.05). In comparison with the control group, the Bmi-1 mRNA expression in the Bmi-1 shRNA group was reduced significantly (P < 0.05). In comparison to the control group, both p16Ink4A and p14ARF mRNA expressions in the Bmi-1 shRNA group were markedly increased (both P < 0.05).. Bmi-1 protein expression in the Bmi-1 shRNA group was shown to be evidently lower than that found in the control group(P < 0.05). In comparison with the control group, both p16Ink4A and p14ARF protein expressions were markedly increased, while both cyclinD 1 and CDK4 protein expressions were significantly reduced in the Bmi-1 shRNA group (all P < 0.05). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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