| Gene name: | FOXO4 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | COLO 829 |
| Experiment: | SA-β-gal activity assay |
| Description: | Ectopic FOXO4 expression rendered Colo829 cells positive for SA-β-GAL activity. |
| Target gene: | BRAFV600E//P21 |
| Official symbol(s): | BRAF//P21 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR//Luciferase reporter assay |
| Target gene description: | We therefore wondered whether BRAFV600E could signal through JNK toward FOXO4 to promote senescence. To fully address this question, we first determined all possible JNK sites of in vitro phosphorylated FOXO4 by liquid chromatography–tandem mass spectrometry analysis.In vitro phosphorylation by JNK significantly increased detection of wild-type FOXO4 by these respective antisera, especially the newly discovered Thr223 and Ser226, whereas FOXO4-4A in which these residues are mutated to Ala was not detected. Together, these results indicate that BRAFV600E promotes JNK-mediated phosphorylation of FOXO4.BRAFV600Ecooperated with FOXO4 to induce p21cip1 expression, and in correlation with the induction of senescence, FOXO4 expression increased p21cip1 expression in Colo829 cells. Similar effects were observed on p21cip1mRNA expression determined by quantitative real-time PCR. Moreover, BRAFV600Eand FOXO4 expression resulted in a synergistic activation of a luciferase-reporter gene driven by the p21cip1promoter. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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