Gene name: | FOXO1 |
Aging type: | Accelerate |
Aging characteristic: |
Tissue type: | -- |
Cell name: | T98G,LN-18 |
Gene ID: | 2308 |
Category: | protein coding |
Phenotype: | Glioblastoma |
Experimental category: | L |
PMID: | 29207098 |
Experiment: | Flow cytometry//MTT assay//Knockdown |
Description: | The results revealed that the overexpression of FOXO1 arrested the cell cycle in the G0/G1 phase, suppressing cells from entering the S phase. The depletion of FOXO1 increased the colony forming ability in cell proliferation of the LN18 and T98G cells. |
Target gene: | SIRT1//P16 |
Official symbol(s): | SIRT1//P16 |
R-AG-Target gene: | Downregulation//Upregulation |
Subcategory: | Unclear |
Target gene experiment: | CHIP//Western blot//qRT-PCR |
Target gene description: | A ChIP assay was performed with FOXO1 antibody, which revealed that the SIRT1 promoter region interacted with FOXO1.In LN18 cells overexpressing FOXO1, the expression of SIRT1 was suppressed.The opposite results were obtained in the FOXO1-depleted cells, which showed SIRT1 was increased and p16INK4a was decreased .The mRNA levels of SIRT1 and p16INK4a were also regulated by FOXO1, which suggested that FOXO1 regulated SIRT1 and p16INK4a at the transcriptional level. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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