| Gene name: | STAT5A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Gene ID: | 100135887 |
| Category: | protein coding |
| Phenotype: | Aging |
| Experimental category: | HL |
| PMID: | 20536843 |
| Experiment: | SA-β-gal activity assay//Cell proliferation assay |
| Description: | We show here that primary MEFs also undergo cell cycle arrest, with markers of cellular senescence when forced to express a constitutively Activate allele of STAT5A (caS5A) or RasV12 by retroviral gene transfer. |
| Regulatory pathway: | JAK-STAT5//P53 |
| R-AG-Pathway: | Activation//Activation |
| Official symbol(s): | hop-STAT5//TP53 |
| Pathway experiment: | SA-β-gal activity assay//PML immunofluorescence//Proliferation assay |
| Pathway description: | To investigate whether we could also trigger senescence by activating JAK/STAT5 signaling upstream of STAT5, we used a Tel/Jak2 fusion protein.54 Similar to caS5A,Tel/Jak2 was also sufficient to trigger senescence cell cycle arrest in MEFs .The process required p53 or the p53 activator p19ARF, because it was totally prevented in MEFs from p53 or p19ARF null mice. |
Annotation:
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