Gene name: | DEK |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | CaSki |
Gene ID: | 7913 |
Category: | protein coding |
Phenotype: | Cervical cancer |
Experimental category: | L |
PMID: | 22390170 |
Experiment: | SA-β-gal activity assay//Knockdown//Flow cytometry//MTT assay |
Description: | Most of the CaSki–DEK and CaSki–IκB cells displayed an intense blue staining, indicative of senescence, whereas only a few positively stained cells were detected in untransfected cells. As measured by microscopic image analysis, the percentage of positive staining was 83.25 +? 9.46% in CaSki–DEK cells and 76.47 +? 6.31% in CaSki–IκB cells,whereas the positive staining in CaSki–neo and untransfected cells was 23.13 +? 7.28 and 18.92 +? 6.46% respectively. The cell growth curve showed that, compared with untransfected and CaSki–neo cells,the proliferation activity of CaSki–DEK cells was inhibited in a time-dependent manner.In CaSki–DEK and CaSki–IκB cells, more cells were blocked in the G0/G1-phase, with a corresponding decrease in the G2/M-phase. The percentage of cells in the G0/G1-phase was significantly increased in CaSki–DEK and CaSki–IκB cells compared with control cells (P < 0.05). |
Target gene: | NF-κBP65 |
Official symbol(s): | NF-κBP65 |
R-AG-Target gene: | Upregulation |
Subcategory: | Unclear |
Target gene experiment: | ELISA//Immunocytochemistry |
Target gene description: | The NF-κB activity determined by ELISA supported the findings observed by immunoblotting. CaSki–DEK and CaSki–IκB cells showed a significant increase in the activity of the NF-κB p65 subunit compared with control cells (P < 0.05). In accordance with this, in CaSki–neo and untransfected cells, NF-κB p65 was retained uniformly in the cytoplasm and only a mild-to moderate expression was observed by immunocytochemical staining. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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