Aging Overview

Aging gene

Gene name: DEK
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: CaSki
Gene ID: 7913
Category: protein coding
Phenotype: Cervical cancer
Experimental category: L
PMID: 22390170
Experiment: SA-β-gal activity assay//Knockdown//Flow cytometry//MTT assay
Description: Most of the CaSki–DEK and CaSki–IκB cells displayed an intense blue staining, indicative of senescence, whereas only a few positively stained cells were detected in untransfected cells. As measured by microscopic image analysis, the percentage of positive staining was 83.25 +? 9.46% in CaSki–DEK cells and 76.47 +? 6.31% in CaSki–IκB cells,whereas the positive staining in CaSki–neo and untransfected cells was 23.13 +? 7.28 and 18.92 +? 6.46% respectively. The cell growth curve showed that, compared with untransfected and CaSki–neo cells,the proliferation activity of CaSki–DEK cells was inhibited in a time-dependent manner.In CaSki–DEK and CaSki–IκB cells, more cells were blocked in the G0/G1-phase, with a corresponding decrease in the G2/M-phase. The percentage of cells in the G0/G1-phase was significantly increased in CaSki–DEK and CaSki–IκB cells compared with control cells (P < 0.05).


Regulatory relationship

Target gene: NF-κBP65
Official symbol(s): NF-κBP65
R-AG-Target gene: Upregulation
Subcategory: Unclear
Target gene experiment: ELISA//Immunocytochemistry
Target gene description: The NF-κB activity determined by ELISA supported the findings observed by immunoblotting. CaSki–DEK and CaSki–IκB cells showed a significant increase in the activity of the NF-κB p65 subunit compared with control cells (P < 0.05). In accordance with this, in CaSki–neo and untransfected cells, NF-κB p65 was retained uniformly in the cytoplasm and only a mild-to moderate expression was observed by immunocytochemical staining.

Regulatory pathway: --
R-AG-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



About risk SNP and eQTL

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