| Gene name: | NOTCH1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay |
| Description: | We examined the replicative lifespan of infected cells and found that up-regulation of Notch1 prolonged the lifespan of endothelial cells along with a decrease of senescence-associated bgalactosidase (SA-β-gal) activity and decreased expression of senescence-associated molecules such as p53, p21, and p16. |
| Regulatory pathway: | ID-1-P16 |
| R-AG-Pathway: | -- |
| Official symbol(s): | ID1-CDKN2A |
| Pathway experiment: | Knockdown//SA-β-gal activity assay//Proliferation assay |
| Pathway description: | To investigate whether Notch1 deletion induced endothelial cell senescence via a p16-dependent pathway, we co-infected human endothelial cells with the p16 shRNA and Notch1 shRNA vectors. Notch1 deletion led to premature senescence of mock-infected cells but not p16 shRNA-infected cells, suggesting that disruption of Notch1 promotes endothelial cell senescence via an Id1/p16-dependent pathway. |
Annotation:
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