Aging Overview

Aging gene

Gene name: NRSN2
Aging type: Accelerate
Aging characteristic:
Tissue type: --
Cell name: SMMC-7721
Gene ID: 80023
Category: protein coding
Phenotype: Hepatocellular carcinoma
Experimental category: L
PMID: 26055238
Experiment: Cell viability assay//SA-β-gal activity assay
Description: We found that when NRSN2 was overexpressed, the cellular viability of SMMC-7721 decreased and the senescent cells increased after doxorubicin treatment for 48 h .


Regulatory relationship

Target gene: BCL-2
Official symbol(s): BCL-2
R-AG-Target gene: --
Subcategory: Unclear
Target gene experiment: Western blot//qRT-PCR
Target gene description: Considering the increased cell viabilities and the anti-senescence/apoptosis effect after NRSN2 silencing, we examined the change of PI3K/Akt pathway, which plays an important role in tumor growth [11] and Bcl-2 family proteins, which function as a critical modulator in cell apoptosis .Meanwhile, the cleaved PARP and pro-apoptotic protein Bax was decreased, while the anti-apoptotic protein Bcl-2 was significantly increased after NRSN2 silencing.

Regulatory pathway: PI3K-AKT-MTOR//P53-P21
R-AG-Pathway: --//--
Official symbol(s): PIK3CA-AKT1-MTOR//TP53-CDKN1A
Pathway experiment: Western blot
Pathway description: Knockdown of NRSN2 led to increased (473)p-Akt levels in MHCC-LM3 cells. After silencing NRSN2 in Hep3B, we treated these transfected cells with doxorubicin, a chemotherapeutic agent which is commonly used to induce cell senescence , for 2 days and we surprisingly found that compared to the control, the levels of p53 and p21 were decreased.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

Loading,please wait...