| Gene name: | MCL1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Human glioma cell |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | The MCL1-siRNA group showed decreased cell density, enlarged body of some cells with flat triangle, and an intense sense of grain, and vacuolation, which were the characteristics of cell senescence, and the positive expression rate of β-galactosidase was (14.26 2.1)% . |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot |
| Pathway description: | Compared with the blank and NC groups, the protein levels of MCL1, Bcl-2, PI3K, Akt, Bmi-1 and extents of PI3K and Akt phosphorylation increased remarkably in the IGF-1 group, and the protein levels of Bax and PTEN decreased significantly ; while the protein levels of MCL1, PI3K, Akt, Bcl-2, Bmi-1 and extents of PI3K and Akt phosphorylation decreased and that of Bax and PTEN elevated significantly in the IGF-1 + MCL1-siRNA group. |
Annotation:
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