| Gene name: | HTRA1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ARPE-19 |
| Experiment: | SA-β-gal activity assay |
| Description: | There were more SA-β-galactosidase-positive cells among HtrA1-expressing ARPE-19 cells (55%) than among control cells (44.3%) after H2O2 treatment. |
| Regulatory pathway: | P38 MAPK |
| R-AG-Pathway: | Activation |
| Official symbol(s): | MAKP14 |
| Pathway experiment: | Western blot//SA-β-gal activity assay |
| Pathway description: | p38 MAPK is activated simultaneously with the HtrA1-enhanced cell senescence in MEFs.To confirm that HtrA1 activates cell senescence through the p38 MAPK cascade, we examined the effects of a p38 MAPK inhibitor, SB203580, on the induction of cell senescence by recombinant HtrA1,SB203580 completely abolished the HtrA1- stimulated expression of p21CIP1/WAF1 and p16INK4a. Similarly, induction of SA-β-galactosidase activity by recombinant HtrA1 was inhibited by SB203580,showing that HtrA1 enhances cell senescence through the p38 MAKP cascade. |
Annotation:
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