| Gene name: | PIM1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | SA-β-gal activity assay |
| Description: | PIM1 induces cell senescence. SA-β-gal activity staining experiments showed strong staining compared to the empty vector control and mPIM1K67M groups. |
| Target gene: | CBX8 |
| Official symbol(s): | CBX8 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | CHIP//CCK-8 assay//EdU assay//Colony formation assay//SA-β-gal activity assay |
| Target gene description: | To further verify whether endogenous CBX8 and PIM1 interact with each other, co-IP experiments were performed in extracts of senescent cells induced by RasV12 as PIM1 expression was upregulated in RasV12-induced senescent cells [9,26]. CBX8 was observed to co-immunoprecipitate with PIM1. In reciprocal immunoprecipitations, PIM1 was observed presenting in CBX8 immunoprecipitates PIM1 overexpression inhibited the growth of 2BS cells, while ectopic expression of CBX8 could obviously mitigate such growth inhibition by PIM1 as indicated in CCK8, EdU incorporation and colony formation assays. Furthermore, SA β-gal activity staining experiment showed that overexpressing CBX8 could make the percentage of stained cells decreasing as compared with that of cells with PIM1 overexpressing alone, which suggests that overexpression of CBX8 may effectively counteract PIM1-induced senescence. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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