| Gene name: | YY1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | H460 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Cells transfected with YY1i exhibited phenotypic changes that resembled those observed in the cells undergoing replicative senescence.These changes include increased SA-β-gal staining, flattened cell morphology, and enlarged cell size. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Acetylation |
| Target gene experiment: | Knockdown//Western blot//CHIP |
| Target gene description: | We used a p16siRNA vector (p16i) to knockdown the endogenous p16 expression [30]. Apparently, compared with the cells transfected with YY1i alone, co-transfection of cells with YY1i and p16i vectors failed to induce cell senescence.Western blotting assays demonstrated that the p16 protein expression was decreased upon overexpression of YY1, while it was enhanced by knockdown of the endogenous YY1 in NCI-H460 cells.The ChIP data revealed that YY1 was enriched at the p16 promoter (P3) upon the overexpression of YY1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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