| Gene name: | BRG1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | We ectopically expressed wild type BRG1 in IMR90 normal human fibroblasts. Indeed, there was a significant increase in SAHF formation in cells ectopically expressing BRG1 compared with controls. Consistently,other markers of SAHF such as formation of H3K9Me2 and mH2A1.2 foci were also induced by the ectopically expressed wild type BRG1.Expression of SA-β-gal activity, a hallmark of cellular senescence, was also induced by wild type BRG1 expression. |
| Target gene: | BRCA1//P16//P21 |
| Official symbol(s): | BRCA1//P16//P21 |
| R-AG-Target gene: | --//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//SA-β-gal activity assay//Western blot |
| Target gene description: | Compared with shBRCA1 alone cells, expression of wild type BRG1 but not mutant BRG1 induced a more pronounced senescence phenotype as indicated by a significant increase in SAHF formation and expression of SA-β-gal activity.This correlated with an increased induction of p16INK4a and P21cip1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...