| Gene name: | RHOA |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HeLa |
| Experiment: | Flow cytometry//SA-β-gal activity assay |
| Description: | Cell cycle analysis (by flow cytometry using PI) of irradiated cells suggested that, after exposure to 15 Gy of γ- radiation, cells with constitutively high levels of activated RhoA (HeLa-RhoA-V14) remain arrested in S and G2/M, whereas HeLa cells expressing the dominant negative RhoAN19 remain predominantly in the G1 and S phases of the cell cycle. As expected, we observed a marked arrest of HeLa cells in the G2/M phase of the cell cycle after treatment with high (15 Gy) dose of γ-radiation .Cells expressing HeLa-RhoAN19, expected to be deficient in RhoA activity, display higher senescence levels at lower doses of 0.5 and 5 Gy of γ-radiation, which correlates with their preferential arrest at G1 and S phases.These cells also showed increased apoptosis 48 and 72 h after exposure to the highest (15 Gy) dose of γ-radiation. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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