| Gene name: | GAPDH |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A549 |
| Gene ID: | 2597 |
| Category: | protein coding |
| Phenotype: | Lung cancer |
| Experimental category: | HL |
| PMID: | 21749859 |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | Knockdown of GAPDH by siGAPDH caused cell proliferation arrest, in contrast to control A549 cells transfected with scrambled siRNA. Cells treated with siGAPDH manifested proliferation arrest, revealed enlarged morphology, and distinct blue staining characteristic for SA-β-galactosidase activation. |
| Target gene: | AMPK//P53 |
| Official symbol(s): | PRKAA2//P53 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown |
| Target gene description: | Following inhibition of glycolysis via GAPDH depletion,the decrease of ATP level resulted in AMPK phosphorylation, and p53 stabilization. In line with these findings, we detected accumulation of p53 phosphorylated at Ser15 in GAPDH knockdown cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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