| Gene name: | UHRF1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDF |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | Compared with DNMT1 knockdown, UHRF1 knockdown more effectively led to the gain of senescent phenotypes. |
| Target gene: | DNMT1//WNT5A |
| Official symbol(s): | DNMT1//WNT5A |
| R-AG-Target gene: | Inhibition//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//qRT-PCR//Western blot//Flow cytometry |
| Target gene description: | Although UHRF1 suppression significantly down-regulated both mRNA and protein expression of DNMT1, DNMT1 knockdown did not alter UHRF1 expression, indicating the role of UHRF1 as an upstream regulator of DNMT1 transcription. However, DNMT1 promoter activity was significantly decreased by siRNA-mediated UHRF1 suppression, further supporting regulation of DNMT1 transcriptional activity by decreased UHRF1 expression. Knockdown of either DNMT1 or UHRF1 clearly induced WNT5A protein expression, whereas HELLS knockdown showed a minor effect.Knockdown experiments revealed that, among the DNMT1-interacting proteins, only UHRF1 knockdown suppressed DNMT1 transcription. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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