| Gene name: | BCL11B |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U87,U251 |
| Experiment: | Knockdown//SA-β-gal activity assay//Cell morphological analysis//PI staining |
| Description: | The histochemical assay for SA-β-Gal activity showed that SA-β-Gal activity was strongly produced in U87-KD1 and U87-KD2 cells with a flat, enlarged cell shape . The extensive SA-β-Gal activity was also detected in U251-KD1 and U251-KD2 cells compared to that of U251-Mock cells. We noted that the activity of SA-β-Gal was weak in either U87- or U251-Mock cells.The percentage of U87-KD1 and U87-KD2 cells at the G0/G1 phase was increased when compared to that observed in U87-Mock cells. In addition, the reduction of Bcl11b expression caused an increase in the arrest of U251 cells at the G0/G1 (KD1) or S (KD2) phase, along with a reduction in the amount of cells at the G2/M phase. |
| Target gene: | P21//P53//SOX2//BMI-1 |
| Official symbol(s): | P21//P53//SOX2//BMI-1 |
| R-AG-Target gene: | --//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | qPCR//Western blot |
| Target gene description: | Similar to p21, the production of p53 mRNA and proteins was increased in C6 and U87 cells after Bcl11b gene knockdown.In addition, the transcripts of the stemness genes, Sox-2 and Bmi-1, were downregulated in U87 and U251 after Bcl11b knockdown, as well as their protein levels.Similar to p21, the production of p53 mRNA and proteins was increased in C6 and U87 cells after Bcl11b gene knockdown. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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