| Gene name: | BMI1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | UCB-MSC |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | As previously reported, BMI1 KD UCB-MSCs exhibited the typical senescent phenotype, such as an enlarged and flattened morphology and increased SA-β-gal activity. |
| Target gene: | MCP-1 |
| Official symbol(s): | CCL2 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown//SA-β-gal activity assay |
| Target gene description: | As expected, the promoters of MCP-1 in BMI1 KD cells lost the recruitment of BMI1 and the concomitant H2AK119Ub histone modification . The epigenetic change in the MCP-1 locus in BMI1 KD cells coincided with derepression of MCP-1 transcription, which resulted in increased secretion of MCP-1 protein. The enhanced secretion of MCP-1 protein in BMI1 KD cells triggered cellular senescence, as judged by the increase in SA-β-gal activity and the activation of the p53-p21 signaling pathway. Most importantly, the senescent phenotypes shown in BMI1 KD cells were significantly rescued after silencing of MCP-1. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//Knockdown//SA-β-gal activity assay |
| Pathway description: | The enhanced secretion of MCP-1 protein in BMI1 KD cells triggered cellular senescence, as judged by the increase in SA-β-gal activity and the activation of the p53-p21 signaling pathway . |
Annotation:
Loading,please wait...